1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in the tissue(chicken, pork,duck), honey, milk, fish, shrimp and egg. The enzyme conjugate is pre-coated on the micro-well stripes. The Benzyl penicillin in the testing sample competes with the conjugate antigens pre-coated on the micro-well stripes, to interact with the antibodies against Chloramphenicol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Benzyl penicillin in it. This value is compared to the standard curve and the content of the corresponding Benzyl penicillin is subsequently obtained.

2. Technical specifications
Sensitivity : 0.1ppb
Detection limit:
Chicken, duck, pork,fish,shrimp ——— 2ppb
Honey, milk ——————————— 2ppb
Recovery rate
Chicken, duck,pork,fish,shrimp ———85%±10%
Honey , milk ———————————70%±10%
Cross-reaction rate:
Benzylpenicillin —————————— 100%
Ampicillin ———————————— 0.8%
Cloxacillin ————————————0.2%
Dicloxacillin ——————————— 0.1%
Amoxicillin ———————————— 0.1%
Ceftiofur ————————————— 0.1%